Severe acute respiratory syndrome coronavirus 3a protein is released in membranous structures from 3a protein-expressing cells and infected cells.
Identifieur interne : 003D12 ( Main/Exploration ); précédent : 003D11; suivant : 003D13Severe acute respiratory syndrome coronavirus 3a protein is released in membranous structures from 3a protein-expressing cells and infected cells.
Auteurs : Cheng Huang [États-Unis] ; Krishna Narayanan ; Naoto Ito ; C J Peters ; Shinji MakinoSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Assemblage viral, Cellules Vero, Cellules cultivées, Humains, Immunoprécipitation, Lignée cellulaire, Plasmides, Protéines de la matrice virale (physiologie), Protéines virales (génétique), Protéines virales (isolement et purification), Protéines virales (métabolisme), Protéines virales (physiologie), Protéines virales structurales (physiologie), Rein (embryologie), Rein (virologie), Syndrome respiratoire aigu sévère (métabolisme), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- embryologie : Rein.
- génétique : Protéines virales, Virus du SRAS.
- isolement et purification : Protéines virales.
- métabolisme : Protéines virales, Syndrome respiratoire aigu sévère.
- physiologie : Protéines de la matrice virale, Protéines virales, Protéines virales structurales, Virus du SRAS.
- virologie : Rein, Syndrome respiratoire aigu sévère.
- Animaux, Assemblage viral, Cellules Vero, Cellules cultivées, Humains, Immunoprécipitation, Lignée cellulaire, Plasmides, Technique de Western.
English descriptors
- KwdEn :
- Animals, Blotting, Western, Cell Line, Cells, Cultured, Chlorocebus aethiops, Humans, Immunoprecipitation, Kidney (embryology), Kidney (virology), Plasmids, SARS Virus (genetics), SARS Virus (physiology), Severe Acute Respiratory Syndrome (metabolism), Severe Acute Respiratory Syndrome (virology), Vero Cells, Viral Matrix Proteins (physiology), Viral Proteins (genetics), Viral Proteins (isolation & purification), Viral Proteins (metabolism), Viral Proteins (physiology), Viral Structural Proteins (physiology), Virus Assembly.
- MESH :
- chemical , genetics : Viral Proteins.
- embryology : Kidney.
- genetics : SARS Virus.
- chemical , isolation & purification : Viral Proteins.
- metabolism : Severe Acute Respiratory Syndrome, Viral Proteins.
- physiology : SARS Virus, Viral Matrix Proteins, Viral Proteins, Viral Structural Proteins.
- virology : Kidney, Severe Acute Respiratory Syndrome.
- Animals, Blotting, Western, Cell Line, Cells, Cultured, Chlorocebus aethiops, Humans, Immunoprecipitation, Plasmids, Vero Cells, Virus Assembly.
Abstract
Severe acute respiratory syndrome coronavirus (SCoV) accessory protein 3a is a virus structural protein. We demonstrate here that 3a protein was released efficiently in membranous structures from various cell lines expressing 3a protein. A subpopulation of the released 3a protein is associated with detergent-resistant membranes. The presence of the YxxPhi and diacidic motifs, located within the cytoplasmic tail of the 3a protein, was not required for its efficient release. Analysis of supernatant from SCoV-infected cells with sucrose gradient sedimentation and virus capture assay indicated that the 3a protein was released from infected cells in two distinct populations, as a component of SCoV particles, and in membrane structures with a lower buoyant density. These data provide new insights into the biological properties of SCoV 3a protein.
DOI: 10.1128/JVI.80.1.210-217.2006
PubMed: 16352545
Affiliations:
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Le document en format XML
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type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name></author><author><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name></author><author><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></author><author><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Immunoprecipitation</term><term>Kidney (embryology)</term><term>Kidney (virology)</term><term>Plasmids</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells</term><term>Viral Matrix Proteins (physiology)</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (isolation & purification)</term><term>Viral Proteins (metabolism)</term><term>Viral Proteins (physiology)</term><term>Viral Structural Proteins (physiology)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Humains</term><term>Immunoprécipitation</term><term>Lignée cellulaire</term><term>Plasmides</term><term>Protéines de la matrice virale (physiologie)</term><term>Protéines virales (génétique)</term><term>Protéines virales (isolement et purification)</term><term>Protéines virales (métabolisme)</term><term>Protéines virales (physiologie)</term><term>Protéines virales structurales (physiologie)</term><term>Rein (embryologie)</term><term>Rein (virologie)</term><term>Syndrome respiratoire aigu sévère (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique de Western</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Rein</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Kidney</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines de la matrice virale</term><term>Protéines virales</term><term>Protéines virales structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Viral Matrix Proteins</term><term>Viral Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Rein</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Kidney</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Cell Line</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Immunoprecipitation</term><term>Plasmids</term><term>Vero Cells</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Humains</term><term>Immunoprécipitation</term><term>Lignée cellulaire</term><term>Plasmides</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SCoV) accessory protein 3a is a virus structural protein. We demonstrate here that 3a protein was released efficiently in membranous structures from various cell lines expressing 3a protein. A subpopulation of the released 3a protein is associated with detergent-resistant membranes. The presence of the YxxPhi and diacidic motifs, located within the cytoplasmic tail of the 3a protein, was not required for its efficient release. Analysis of supernatant from SCoV-infected cells with sucrose gradient sedimentation and virus capture assay indicated that the 3a protein was released from infected cells in two distinct populations, as a component of SCoV particles, and in membrane structures with a lower buoyant density. These data provide new insights into the biological properties of SCoV 3a protein.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name><name sortKey="Narayanan, Krishna" sort="Narayanan, Krishna" uniqKey="Narayanan K" first="Krishna" last="Narayanan">Krishna Narayanan</name><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Huang, Cheng" sort="Huang, Cheng" uniqKey="Huang C" first="Cheng" last="Huang">Cheng Huang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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